Testosterone replacement maintains smooth muscle content in the corpus cavernosum of orchiectomized rats

doi:10.1016/j.ajur.2017.02.001

Asian Journal of Urology, 2017, 4 (4): 223-229 doi: 10.1016/j.ajur.2017.02.001

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Testosterone replacement maintains smooth muscle content in the corpus cavernosum of orchiectomized rats

Graziele Halmenschlager^a, Ernani Luis Rhoden^b, Gabriela Almeida Motta^c, Lucas Sagrillo Fagundes^d, Jorge Luiz Medeiros Jr^e,f, Rosalva Meurer^c, Cláudia Ramos Rhoden^c

a Centro Universitário Ritter dos Reis (UniRitter) and Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA)-PPG Ciências da Saúde, Porto Alegre, RS, Brazil;
b Department of Urology at Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA), Urologist at Irmandade Santa Casa de Misericórdia de Porto Alegre (ISCMPA) and Hospital Moinhos de Vento de Porto Alegre (HMV), Porto Alegre, RS, Brazil;
c Universidade Federal de Ciências da Saúde de Porto Alegre (UFCSPA)-PPG Ciências da Saúde-Porto Alegre, RS, Brazil;
d INRS Institut Armand Frappier (Universit. du Québec) and BioMed Research Center Laval, Quebec, Canada;
e Laboratory of Morphometry, Metabolism, and Cardiovascular Disease, Biomedical Center, Institute of Biology, State University of Rio de Janeiro, Rio de Janeiro, Brazil;
f Laboratory of Multidisciplinary of Sciences, Biomedical Center, Medical School of Valença, Valença/Rio de Janeiro, Brazil

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Abstract Objective: To evaluate the effects of testosterone (T) on the maintenance of corpus cavernosum (CC) structure and apoptosis.
Methods: Animals were divided into three groups:sham operation group (n=8) underwent sham operation; Orchiectomized (Orchiec)+oily vehicle group (n=8) underwent bilateral orchiectomy and received a single dose of oily vehicle by intramuscular injection (i.m.) 30 days after orchiectomy; and Orchiec+T group (n=8) underwent bilateral orchiectomy and received a single dose of T undecanoate 100 mg/kg i.m. 30 days after the surgery. Animals were euthanized 60 days after the beginning of the experiment with an anesthetic overdose of ketamine and xylazine. Blood samples and penile tissue were collected on euthanasia.Azan's trichrome staining was used to evaluate smooth muscle,Weigert's Fucsin-Resorcin staining was used to evaluate elastic fibers and Picrosirius red staining was used to evaluate collagen.Apoptosis was evaluated using TUNEL technique.
Results: T levels decreased in Orchiec + oily vehicle when compared to sham operation and Orchiec + T groups (p < 0.001).T deprivation reduced trabecular smooth muscle content and penile diameter and T replacement maintained both parameters (p = 0.005 and p = 0.001,respectively).No difference was observed in the content of sinusoidal space (p = 0.207),elastic fibers (p = 0.849),collagen (p = 0.216) and in apoptosis (p= 0.095).
Conclusion: Normal testosterone levels maintain CC smooth muscle content and do not influence elastic fibers,collagen content and apoptotic index.Further studies should be performed in order to investigate the mechanisms by which androgen mediates its effects on CC structure.

Key words： Testosterone replacement Histomorphometry Androgen deprivation Hypogonadism Corpus cavernosum

Received: 06 May 2016 Published: 26 October 2017

Fund:We thank Urogenital Research Unit staff, UERJ, for technical support and UFCSPA, CNPq (MCT/CNPq 15/2007), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-CAPES and FAPERGS (11/1003-5-02/2011) for financial support.

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	Graziele Halmenschlager
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	Lucas Sagrillo Fagundes
	Jorge Luiz Medeiros Jr
	Rosalva Meurer
	Clá
	udia Ramos Rhoden

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Graziele Halmenschlager,Ernani Luis Rhoden,Gabriela Almeida Motta, et al. Testosterone replacement maintains smooth muscle content in the corpus cavernosum of orchiectomized rats[J]. Asian Journal of Urology, 2017, 4(4): 223-229.

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http://www.ajurology.com/EN/10.1016/j.ajur.2017.02.001 OR http://www.ajurology.com/EN/Y2017/V4/I4/223

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